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Acheter zithromax monodose syringe (Monofil, Germany). Antibiotic tablets were administered at 0, 1, 3 and 7 days, according to the therapeutic regimen. Study Design The study protocol was written by the institutional review board of University Hospital Basel (Consent Nos. No. 08/A/06/01). All animal studies were approved by the Swiss Federal Office for Research and Technology (NO. 4.085/2014) the Swiss Federal Office for Consumer Protection. The study was conducted in accordance with the Declaration of Helsinki (Supplementary Material, available at NEJM.org). The study was conducted in collaboration with the Biotechnology and Chemical Sciences Centre of the University Basel and Basel-based Institut für Technische Forschung. An institutional ethics committee at Basel and the German Federal Institute for Risk Assessment approved the study (No. 008.00.003). Animals Thirty-one CD-1 mice were randomly selected and housed in a veterinary clinic (B. Braun-Universität Basel) and maintained on a 12-h light/dark cycle at 21 °C with a light/dark cycle of 12 h/12 h. The mice were fed an experimental diet and were under observation once a week. The experimental diet containing 50% of energy from carbohydrates, protein and fats differed from a standard mouse diet in containing low (2%) or high (100%) amounts of nonstarch polysaccharides, which were supplemented according to the study protocol (Supplementary Material, available at NEJM.org). The total animal weight was estimated from the of tail. The pharmacotherapeutic regimen used in human and animal trials was as follows: i) antibiotics, given during the first 4 h of light/dark cycle, were given after an appropriate pretreatment; ii) antibiotics were continued in a titrated fashion every 4 h for a total of 20 g antibiotics; iii) the mice were euthanized by CO 2 when the average clinical score reached 50% or 80%, with an interval of one day between each euthanasia; iv) all body weights were recorded every day; v) the body weights of all mice were weighed upon removal from the apparatus. clinical scores and body measurements performed at the end of study were compared at regular intervals and changes in clinical score (and therefore the therapeutic dose of antibiotics given in this study) could be defined and compared between the mice, mice whose mortality rate was below a specified maximum and mice with high mortality rates. If it was evident that the mice showed severe clinical lesions, the body weights of any mice were excluded from the analysis. Chemotherapy Treatment Preparations The chemotherapeutic dosage for kmart pharmacy generic drug price list human and animal studies was based on previous published data obtained in the human population (12). chemotherapeutic regimen was as follows: i) 15% salmeterol at a dose of 3 mg/kg in the right flank for 24 h; ii) 3 days of 20% salmeterol i.v. for 6 days; iii) 3 days of 10% tetracycline i.v. for 3 days; iv) days of 0.1% methylprednisolone i.v. for 3 days; and v) days of 0.05% gentamicin i.v. for 3 days. The therapeutic dosage for human studies was based on previous published data obtained in humans (12), but was slightly more than that proposed according to the current guidelines (12). For our studies we used a regimen that is less severe than recommended according to the current guidelines, because we thought that a short hospital stay might increase the possibility of progression to liver failure. The doses used in mouse studies this study were higher than those used with respect to time the clinical onset of disease in humans, because the lower body weights of mice being assessed in this study compared to those in the human studies, and because mice were considered to be in a more favorable position due to a shorter interval between experimental injections compared to humans. It should also be noted that the maximum dose of salmeterol used in this study was only 30 mg/kg, which would have been too high for the mice in our mouse study, which had a maximum dose of 10 mg/kg. The human experiments involved 5 subjects, divided into groups of n = 5. Because the possible adverse effects of long-term treatment with salmeterol, and because the concentrations in human experiments were higher than those used in the mouse studies, concentration used in the mouse studies (0.2%) was used for all groups, except 2 of the 5 subjects who received salmeterol alone and were administered a 4-wk treatment with 3 mg/kg or per week of salmeterol, 4 mg/kg salmeter.
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